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$39.95Category: Storage & Reconstitution
Lyophilized (freeze-dried) peptides should be stored at minus 20 degrees Celsius or colder, in their original sealed amber glass vial, protected from light and moisture. Under those conditions, lyophilized peptides typically maintain 99%+ HPLC purity for 24 to 48 months. Full storage detail and shelf-life tables are at reconstitution storage.
Once reconstituted with bacteriostatic water, peptide solutions should be refrigerated at 2 to 8 degrees Celsius and used within the shelf life noted on the product page (typically 1 to 8 weeks depending on peptide chemistry). Do not freeze reconstituted solutions unless aliquoted for single-use. Full reconstituted shelf-life tables are at reconstitution storage.
Inject the calculated volume of bacteriostatic water slowly down the inside wall of the vial so the stream does not strike the lyophilized pellet. Swirl gently for 30 to 60 seconds. Do not shake. Most standard peptides should fully dissolve within this window. For exact bacteriostatic water volumes, use the calculator. For the full step-by-step protocol, see reconstitution storage.
Bacteriostatic water is sterile water with 0.9% benzyl alcohol added as a preservative. Benzyl alcohol inhibits bacterial growth in a multi-dose vial, allowing the reconstituted solution to be used within its refrigerated shelf life. Bacteriostatic water is the default solvent for most research peptides.
Standard hydrophilic peptides reconstituted in bacteriostatic water typically remain stable for 4 to 8 weeks at 2 to 8 degrees Celsius. Peptides with free cysteine or methionine residues typically hold 1 to 2 weeks. Hydrophobic peptides reconstituted in acetic acid typically remain stable for 2 to 4 weeks. Specific shelf life by peptide class is on reconstitution storage.
Freezing slows degradation, but each freeze-thaw cycle costs 1-5% of purity, depending on peptide chemistry. Best practice is to aliquot the reconstituted vial into single-use volumes before freezing, so each aliquot is thawed once. Avoid freeze-thawing the same aliquot twice. Detailed aliquot strategy is at reconstitution storage.
A 1 mL U-100 insulin syringe is the standard choice for most research draws. For very small draws (below 5 IU on the U-100 scale), a 0.3 mL or 0.5 mL syringe gives finer resolution. The calculator returns the exact draw volume in both mL and IU for any vial size and target dose.
Avoid four common mistakes: vigorous shaking during reconstitution, injecting bacteriostatic water directly onto the lyophilized pellet, storing reconstituted solution at ambient temperature, and freeze-thawing the same aliquot more than once. The full list of mistakes to avoid is on reconstitution storage.
